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. Author manuscript; available in PMC: 2018 Jan 1.
Published in final edited form as: Methods Enzymol. 2016 Dec 14;582:137–169. doi: 10.1016/bs.mie.2016.10.036

Table 1.

Oligonucleotides for Constructing Hairpin

Oligonucleotide Sequence (IDT Format, 5′ to 3′)
LH forward primer /5Biosg/TGA AGT GGT GGC CTA ACT ACG
LH reverse primer CAA GCC TAT GCC TAC AGC AT
RH forward primer /5Phos/TTG AAA TAC CGA CCG CTC AGC TAT CAG CCT TTT TTT TTT TTT TTT TTT/idSp/CTC TGA CAC ATG CAG CTC CC
RH reverse primer /5DigN/CAA CAA CGT TGC GCA AAC T
Hairpin insert /5Phos/CCT GGG GCT GAT AGC TGA GCG GTC GGT ATT TCA AAA GTC AAC GTA CTG ATC ACG CTG GAT CCT AGA GTC AAC GTA CTG ATC ACG CTG GAT CCT ATT TTT AGG ATC CAG CGT GAT CAG TAC GTT GAC TCT AGG ATC CAG CGT GAT CAG TAC GTT GAC TT