Skip to main content
. Author manuscript; available in PMC: 2017 Sep 20.
Published in final edited form as: Oncogene. 2017 Mar 20;36(29):4089–4099. doi: 10.1038/onc.2017.11

Fig. 6. Increased iron efflux reduces proliferation of COV362 ovarian cancer cells.

Fig. 6

(a) q-RTPCR of FPN (normalized to βactin) and immunofluorescence staining of FPN in COV362 and HOSE cells: FPN in red; nuclei in blue. Scale bar 20 μm. (b)q-RTPCR of TFR1/βactin in COV362 ovarian cancer cells and HOSE cells; (c) FPN was induced at time 0 by the addition of doxycycline and cell viability assessed at the indicated timepoints by MTS assay; (e) Colony formation of COV362cells with and without ferroportin overexpression was analyzed by crystal violet staining. Colonies from three replicate wells were counted and quantified.