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Circular dichroism (A) and visible absorption (B) spectra comparing FeS-fIscU (red) and FeS-yIsu1 (blue). Both fIscU and yIsu1 have similar CD features to an archetypical 2Fe-2S cluster from the yeast ferredoxin, Yah1 (black). All spectra represent 50 μM protein in a 1-cm path-length cuvette.
