Extended Data Fig. 5. Dopamine release from human T_FH cells.

a, Bar plot showing dopamine release from T_FH cells after 30 min stimulation with autologous B cells (1:2) alone or with anti-CD3/CD28 beads (1:1). T_FH cells were pre-stimulated with forskolin before inducing DA release. Bars represent median and each dot represent a single experiment conducted in triplicates (n=4). b, Bar plot showing dopamine release from T_FH cells after 30 min stimulation with allogeneic GC B cells (1:2) alone or in the presence of ICOSL blocking antibody (10 μg/ml). T_FH cells were pre-stimulated with forskolin before inducing DA release and B cells were pre-stimulated with 10 μM DA to increase ICOSL surface levels before incubation with T_FH cells. Bar represent median of DA level in T_FH cells (n=3) and each triangle represent allogeneic B cells from a single donor paired with its control (square, n=11). *p ≤ 0.05; paired t-test.