a, Gating strategy for sorting T cell subsets from human tonsil.
b, Quantification of catecholamines by GC/MS/MS (n=2).
c, Representative dopamine FACS stain. d,
Quantification of DA-expressing cells (n=3). e, Representative
immunofluorescence dopamine stains of TFH cells untreated or treated
with forskolin (FSK) (n=5). f, DA expression in untreated or
FSK-treated TFH and non-TFH (effector and naïve T).
(n=5). g, Representative GC/MS/MS peaks showing DA content in
FSK-treated and untrated TFH cells compared to internal standard
(13C6-labelled DA), which controls for losses during
extraction. h, CHGB and TH mRNA
expression by qPCR (normalised to RPL13A) in FSK-treated
TFH and non-TFH cells. r.u., relative unit. (n=3).
f,h, Bars represent median values; each dot
represents one donor. ns, not significant and **p ≤ 0.01; Mann-Whitney
test.