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. Author manuscript; available in PMC: 2018 Jan 12.
Published in final edited form as: Nature. 2017 Jul 12;547(7663):318–323. doi: 10.1038/nature23013

Fig. 2. Human TFH cells produce dopamine.

Fig. 2

a, Gating strategy for sorting T cell subsets from human tonsil. b, Quantification of catecholamines by GC/MS/MS (n=2). c, Representative dopamine FACS stain. d, Quantification of DA-expressing cells (n=3). e, Representative immunofluorescence dopamine stains of TFH cells untreated or treated with forskolin (FSK) (n=5). f, DA expression in untreated or FSK-treated TFH and non-TFH (effector and naïve T). (n=5). g, Representative GC/MS/MS peaks showing DA content in FSK-treated and untrated TFH cells compared to internal standard (13C6-labelled DA), which controls for losses during extraction. h, CHGB and TH mRNA expression by qPCR (normalised to RPL13A) in FSK-treated TFH and non-TFH cells. r.u., relative unit. (n=3). f,h, Bars represent median values; each dot represents one donor. ns, not significant and **p ≤ 0.01; Mann-Whitney test.