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. Author manuscript; available in PMC: 2018 Jan 12.
Published in final edited form as: Nature. 2017 Jul 12;547(7663):318–323. doi: 10.1038/nature23013

Fig. 4. Dopamine induces ICOSL upregulation on human GC B cells.

Fig. 4

a, Gating of GC B cells and fluorescence intensity of specified proteins 30 minutes after stimulation with DA (10μM) (n=3). b, Fold changes of surface ICOSL expression with medium control set as unit 1 (n=8). c, Survival of GC B cells after DA stimulation (n=8). d, Fold changes of surface ICOSL expression on GC B cells stimulated with DA (10μM), DA agonist SKF38393 (10nM), Haloperidol (50nM) and DA antagonist SKF83566 (10nM) for 30 min, with medium control set as unit 1 (n=5). e, f, Representative histograms (e) and quantification (f) of surface and intracellular ICOSL on naïve, memory and GC B cells (n=4); Mann-Whitney test. g, RNA counts per million (CPM) of indicated transcripts in human GC B cells stimulated with or without DA (5μM) for 2h (n=3). h, Fold changes of surface ICOSL expression on human GC B cells treated with cycloheximide (CHX, 10 μg/ml) and stimulated with DA (10μM) for 30 min. i, j, Fold changes of surface ICOSL expression on human GC B cells stimulated with DA (10μM), anti-CD40 (1 μg/ml) or recombinant CD40L (10 μg/ml) (i), IL-21 (10, 50 or 100 ng/ml) or IL-4 (10 νγ/ml) (j) for 30 min (n=5). b, d, h-j, Bars represent medians and each dot represents a single experiment conducted in triplicate (n=10); two tailed student t-test. ns, not significant, *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001 and ****p ≤ 0.0001.