a, Gating of GC B cells and fluorescence intensity of specified
proteins 30 minutes after stimulation with DA (10μM) (n=3).
b, Fold changes of surface ICOSL expression with medium control
set as unit 1 (n=8). c, Survival of GC B cells after DA stimulation
(n=8). d, Fold changes of surface ICOSL expression on GC B cells
stimulated with DA (10μM), DA agonist SKF38393 (10nM), Haloperidol (50nM)
and DA antagonist SKF83566 (10nM) for 30 min, with medium control set as unit 1
(n=5). e, f, Representative histograms
(e) and quantification (f) of surface and
intracellular ICOSL on naïve, memory and GC B cells (n=4); Mann-Whitney
test. g, RNA counts per million (CPM) of indicated transcripts in
human GC B cells stimulated with or without DA (5μM) for 2h (n=3).
h, Fold changes of surface ICOSL expression on human GC B cells
treated with cycloheximide (CHX, 10 μg/ml) and stimulated with DA
(10μM) for 30 min. i, j, Fold changes of surface ICOSL
expression on human GC B cells stimulated with DA (10μM), anti-CD40 (1
μg/ml) or recombinant CD40L (10 μg/ml) (i), IL-21
(10, 50 or 100 ng/ml) or IL-4 (10 νγ/ml) (j) for 30
min (n=5). b, d, h-j, Bars represent medians and each dot
represents a single experiment conducted in triplicate (n=10); two tailed
student t-test. ns, not significant, *p ≤ 0.05, **p ≤ 0.01, ***p
≤ 0.001 and ****p ≤ 0.0001.