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. Author manuscript; available in PMC: 2017 Aug 2.
Published in final edited form as: J Cell Physiol. 2015 Nov 24;231(6):1291–1300. doi: 10.1002/jcp.25229

Fig. 5.

Fig. 5

Inhibition of PI3Ks prevented the inhibitory effects of cAMP and cGMP agonists on directional migration of cell fragments. (A) Inhibition of PI3Ks with LY294002 (50 μM) completely recovered directional migration of cell fragments treated with cAMP and cGMP agonists. Inhibition of Myosin II with blebbistatin (BB; 50 μM) had no effect. (B) Inhibition of PI3Ks and myosin II did not affect the migration speed of cell fragments. Data are shown as mean ± s.e.m. from 30 to 80 fragments; the results were confirmed in three independent experiments. EF = 400 mV/mm; duration, 30 min. Fragments were treated with inhibitors and/or agonists for 30 min before experiments. *P <0.05; **P <0.01. NS: no significant difference.