Table 1. Measuring range and precision.

Analyte	LoD, ng/mL	LLoQ, ng/mL	ULoQ, ng/mL	Inter-run CV	Intra-run CV
CTSD	1.1	5.2	1’125	6.2% (3.9–9.8%)	4.2% (2.8–6.1%)
ICAM1	1.7	12.0	600	4.2% (2.3–5.5%)	6.7% (5.6–8.3%)
OLFM4	2.1	14.8	3’200	13.0% (10.6–19.7%)	7.7% (3.4–14.6%)
THBS1	102	852	368’000	4.2% (3.3–4.8%)	10.5% (9.4–12.5%)

LoD was calculated by adding 3 SD to the mean of 24 blanks. Acceptance criterion for LLoQ and ULoQ was a CV of <25% and <20%. Inter CV was calculated by comparing triplicate measurements of 11 serum samples on 20 different runs over 5 days. Intra CV was defined by measuring 5 serum samples in 18 replicates.