Table 2.

shows the individual detached cell transfections performed that are summarized in Fig. 5a–c with information about the transfected construct, the parasite line that was transfected, the type of integration, the success rate, how often the transfection was performed and at which day after the transfection the mice became positive

Construct	Parasite line	Integration	Success rate	Number of transfections	Day mice became positive
PbSBP1KO (P0B90-GFP) [19]	mCherryHsp70 [20]	Double crossover	50% (2)	2	7
PbSBP1KO (P0B90-GFP) [19]	mCherryHsp70FLucef1a [21]	Double crossover	100% (1)	2	6
PbSBP1KO (P0B90-GFP) [19]	WT	Double crossover	100% (1)	1	7
PfSBP1 (PL0017-mCherry) [19]	PbSBP1KO [19]	Single crossover	100% (1)	1	8
PbGEM-336027	mCherryHsp70 [20]	Double crossover	100% (1)	1	21
PbNLuc (PL0017) [18]	WT	Single crossover	100% (1)	1	5
PbNLuc (PL0017) [18]	mCherryHsp70 [20]	Single crossover	100% (2)	2	4
PbNLuc (PL0017) [18]	mCherryHsp70FLucef1a [21]	Single crossover	66.6% (3)	3	4–5
PL0017 GFP [22]	mCherryHsp70 [20]	Single crossover	100% (8)	8	5–12