Peritoneal and circulating numbers of neutrophils after injection of chemokines immediately following CLP. Mice were injected i.p. with saline (200 μl) or chemokines (500 ng CXCL2 + 50 ng CXCL1). The i.p. injection of chemokines increased the early, 6 h, recruitment of neutrophils into the peritoneum. For A, C, at 6 h n = 10 for saline and 10 for chemokines, and at 24 h n = 20 for saline and 19 for chemokines. For B, at 6 h n = 9 for saline and 7 for chemokines, and at 24 h n = 27 for saline and 26 for chemokines. Results are shown as mean ± SEM. p < 0.05 when compared with saline at 6 h (a); chemokines at 6 h (b); saline at 24 h (c).