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. Author manuscript; available in PMC: 2017 Aug 2.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2015 Sep 17;35(11):2374–2383. doi: 10.1161/ATVBAHA.115.305879

Figure 1. DNmDia expression inhibited SM-specific marker expression.

Figure 1

A) Schematic of full-length and dominant negative mDia1. FH, formin homology domain; GBD, GTPase-binding domain; DID, diaphanous inhibitory domain; DAD, diaphanous autoinhibitory domain. B) 10T1/2 cells transfected with DNmDia or empty expression vector were serum-starved, and treated with sphingosine-1-phosphate (S1P) for 16 hr. Endogenous SMC marker expression was detected by immunoblotting. C) 10T1/2 cells expressing mCherry empty vector (EV) or mCherry-DNmDia were serum-starved, treated with S1P for 4 min, and then fixed. Localization of endogenous MRTF-A was determined by immunohistochemistry. D) Quantification of MRTF-A nuclear localization from three separate S1P-treated experiments with over 100 cells counted per condition. * p < 0.05.