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. Author manuscript; available in PMC: 2017 Aug 2.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2015 Sep 17;35(11):2374–2383. doi: 10.1161/ATVBAHA.115.305879

Figure 6. Induction of DNmDia in adult mice inhibited SMC differentiation marker gene expression.

Figure 6

Control and DNmDia+/SMMHCCre+ mice were injected with tamoxifen IP for 5 consecutive days. The indicated tissues were harvested after 3 weeks, and frozen sections were immunostained for SM MHC. A) Representative images and quantification of SM MHC staining. B) Represenative Western blots for DNmDia and SM MHC expression in aorta, bladder, and stomach from WT and DNmDia+/SMMHCCre+ mice, three weeks after tamoxifen treatment. C) SM MHC expression relative to α-tubulin was quantified using image J and then normalized to expression in WT animals set to 1. n=at least 9 animals per genotype. *p < 0.05.