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. Author manuscript; available in PMC: 2017 Aug 2.
Published in final edited form as: Dev Neurobiol. 2013 Nov 20;74(4):457–466. doi: 10.1002/dneu.22136

Figure 3.

Figure 3

Pruning of fibers in the inner hair cell and outer hair cell areas. A, B: Hair cell synapses, stained for neurofilament (NF, green), PSD95 (red), and CtBP2 (blue). Arrows indicate triple-stained synapses (NF, PSD95 and CtBP2) in the outer (A) and inner (B) hair cell areas. Arrowheads (in A) indicate the areas (a and b) shown at higher power to the right. Box (in B) indicates area enlarged to the right. C, D: Up to 8% of the hair cells, mostly inner hair cells, were contacted after 4–10 DIV by spiral ganglion neurons (C). Quantification of synapses indicated that the number of hair cells innervated by a single neuron decreased with increasing time in culture toward a value of 1.5 (D) (Scale bars, 5 µM). [Color figure can be viewed in the online issue, which is available at wileyonlinelibrary.com.]