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. 2017 Aug 2;12(8):e0182291. doi: 10.1371/journal.pone.0182291

Fig 1. Induced LSR and TRIC expression in temperature-sensitive mouse cochlear cells.

Fig 1

(A) LSR and TRIC immunocytochemistry of cells incubated at 33°C and 39°C. Scale bar: 20 μm. (B) Western blotting and (C) RT-PCR analysis of LSR and TRIC expression after 1 and 2 days incubated at 39°C. (D) LSR and TRIC immunocytochemistry of cells incubated at 33°C and 39°C after treatment with 100 μM metformin. Scale bar: 20 μm. (E) Western blotting for phospho-AMP kinase (pAMPK) in cells cultured at 33°C treated with 10 or 50 μM metformin. (F) Western blotting and (G) RT-PCR for LSR and TRIC expression in cells incubated at 39°C treated with 10 or 100 μM metformin.