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(A) Immunocytochemical staining for ZO-1, LSR, TRIC, and Ac-tubulin in differentiated cochlear cells after TSA and iHDAC6 treatment. Scale bar: 20 μm. (B) Double-immunocytochemistry for ZO-1 and LSR in differentiated hair cells after TSA. Scale bar: 20 μm. (C) Western blotting for ZO-1 after treatment with 0.1 or 1 μM TSA and 0.1 or 1 μM iHDAC6.
