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. 2017 Aug 2;13(8):e1006530. doi: 10.1371/journal.ppat.1006530

Fig 1. CD40 on DCs is required for the generation of antigen-specific IL-17 responses during Mtb infection.

Fig 1

(A) DCs derived from B6 or CD40-/- mice were pulsed with OVA323-339 at the indicated concentrations and infected with Mtb for 24 hours followed by co-culture with purified OT-II TCR-Tg CD4 T cells for 72 hours. Supernatants were assayed for the indicated cytokines by ELISA. (B) DCs from B6, CD40-/-, or CD80-/-CD86-/- mice were pulsed with 10 μg/ml OVA323-339, infected with Mtb and co-cultured with OT-II TCR-Tg CD4 T cells. Cell-free supernatants were harvested after 72 hours and assessed for the indicated cytokines by ELISA. (C) B6 or CD40-/- DCs were left uninfected (UI) or infected with Mtb. After 24 hours, cell-free supernatants were collected and assessed for the indicated cytokines by ELISA. Data are representative of 3–4 independent experiments. Values are presented as mean ± SD. Statistical significance was determined using a 2-tailed unpaired T-test. * p<0.05; ** p<0.005, *** p<0.0005, **** p<0.0001, ns = not significant.