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. 2017 Aug 2;12(8):e0182389. doi: 10.1371/journal.pone.0182389

Fig 4. Clusterin expression was examined by immunoblot analysis in RP Saline, RP Saline (Rt), and RP Clusterin (Lt) retinas.

Fig 4

Clusterin or saline was injected at P15 of RP, and then the retinas were collected at 5 minutes, 30 minutes, 1 hour, 6 hours, 24 hours, and 48 hours. A significant increase in clusterin (i.e. clusterin precursor and clusterin-α) was detected as early as 5 minutes in both RP Clusterin (Lt) and RP Saline (Rt) compared to that of 5minutes RP Saline retinas. The expression of clusterin did not change among the RP saline groups (i.e. 5 minutes, 30 minutes, 1 hour, 6 hours, 24 hours, and 48 hours, data not shown). Furthermore, the expression of clusterin was higher in the RP Clusterin (Lt) retinas than in the RP Saline (Rt) at 1hour after clusterin injection (A). Densitometry analysis of clusterin precursor (B) and clusterin alpha subunit (C) was shown by measuring the intensity relative to control β-actin (B, C). Data represents mean ± SEM, **** P<0.0001.