Skip to main content
. Author manuscript; available in PMC: 2017 Aug 2.
Published in final edited form as: Mol Cell. 2016 Apr 21;62(2):194–206. doi: 10.1016/j.molcel.2016.03.036

Figure 3. Histone β-Hydroxybutyrylation is Metabolically Regulated by Cellular β-Hydroxybutyrate Levels.

Figure 3

(A) Immunoblot analysis of histones from S. cerevisiae, D. melanogaster S2 cells, MEF cells, and HEK293 cells using pan anti-Kbhb antibody.

(B) Immunoblot analysis of histones from HEK293 cells treated with dose-increased sodium β-hydroxybutyrate.

(C) Blood glucose and β-hydroxybutyrate concentrations measured by a glucose-ketone meter from “fed” or “fasted” mice. Data are represented as means (±SEM). Twenty “fed” and 30 “fasted” mice were used. **P < 0.01, ***P < 0.001.

(D) Histone Kbhb and Kac levels in livers from “fed” or “fasted” mice were detected by Western blot using indicated antibodies.

(E) Blood glucose and β-hydroxybutyrate concentrations measured from “healthy” or “STZ-treated” mice. Data are represented as means (±SEM). Six pairs of healthy and streptozotocin (STZ) treated mice were used. **P < 0.01, ***P < 0.001.

(F) Histone Kbhb and Kac levels in livers from “healthy” or “STZ-treated” mice were detected by Western blot using indicated antibodies. See also Figure S3.