Skip to main content
. Author manuscript; available in PMC: 2017 Oct 10.
Published in final edited form as: Cancer Cell. 2016 Oct 10;30(4):563–577. doi: 10.1016/j.ccell.2016.09.005

Figure 6. N-MYC increases AKT signaling in cell line and organoids.

Figure 6

A Western blot analysis of p-AKT in LNCaP-NMYC cells compare to LNCaP-cntl (upper panel). Western blot analysis of p-AKT and Phospho-S6 Ribosomal Protein (Ser235/236) in LNCaP-NMYC cells compare to LNCaP-cntl, 24 hours after treatment with BKM120 (1 μM) and RAD001 (100 nM) alone or in combination (middle panel). Dose-response following 72-hour incubation with the indicated dose of BEZ235 obtained for LNCaP-NMYC cells and LNCaP-cntl and in the presence or absence of MLN8237 (100 nM) (lower panel). B. Drug treatment in prostate organoids culture. Top: Experiment design and below: Western blot analysis of p-AKT after 48 hours of BKM120 (1 μM) and BEZ235 (100 nM) treatment. Bottom: Dose response of BKM120 for T2-Cre+/+; Ptenf/+; LSL-MYCN+/+ organoid culture compare to the non-induced organoids. See also Figure S6.