Figure 6. Fusion of proteoliposomes with Qc-3Δ.
(A) Fusion reactions were performed with ATPγS as in Figure 3A, but with Qc-3Δ instead of Qc. (B) Fusion reactions were performed as in part A, but with ATP instead of ATPγS. Sec17 was wild type (wt) or had the mutations F21S,M22S (FSMS), K159E,K163E (KEKE), or L291A,L292A (LALA). (C) Fusion with Qc-3Δ needs the Sec17 apolar loop for more than membrane anchoring. Fusion with ATPγS and Qc-3Δ was with proteoliposomes bearing TM-Sec17 wild-type or TM-Sec17 F21S,M22S, and with 16 nM HOPS (open symbols) or 100 nM HOPS (filled symbols), as indicated.
DOI: http://dx.doi.org/10.7554/eLife.26646.021
Figure 6—source data 1. Source data file (Excel) for Figure 6 Parts A and B.
elife-26646-fig6-data1.xlsx (77.7KB, xlsx)
DOI: 10.7554/eLife.26646.022
Figure 6—source data 2. Source data file (Excel) for Figure 6C.
elife-26646-fig6-data2.xlsx (65.8KB, xlsx)
DOI: 10.7554/eLife.26646.023
Figure 6—figure supplement 1. Average and standard deviations of fusion after 30 min for triplicate assays as in Figure 6C, relative to the maximal fusion condition in panels A and C, and as % lumenal compartment mixing in panel B.
