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. 2017 Jul 21;6:e27564. doi: 10.7554/eLife.27564

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© 2017, Ramond et al

This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

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Figure 1. — (A) The flow cytometry plot represents CD45 and CD31 expression against EPCAM gated on live human fetal pancreatic cells (9.7WD), n = 9. (B) Immunohistochemistry for PDX1 and EPCAM on pancreatic section (9WD), n = 3. Scale bar = 100 μm. (C–E) Flow cytometry plots of PDX1 and NKX6-1 expression at 9.4WD on CD45⁺/CD31⁺ cells (red square), CD45^-CD31^-EPCAM^- cells (TN = triple negative green square) and CD45^-CD31^-EPCAM⁺ cells (blue square). (F) RT-qPCR analysis of PDX1 and NKX6-1 expression on sorted CD45^-CD31^-EPCAM⁺ and TN cells. ND = Not Detected.

DOI: http://dx.doi.org/10.7554/eLife.27564.002