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. 2017 Aug 2;12(8):e0182278. doi: 10.1371/journal.pone.0182278

Fig 3. Generation of vinculin A-deficient zebrafish mutants using TALENs.

Fig 3

(A) Schematic representation of the endogenous vcla locus targeted by TALEN gene editing technology at the exon4-intron4 boundary. The TALEN arms flank a BclI restriction enzyme recognition site (highlighted in red) used for screening mutant alleles through restriction fragment length polymorphism (RFLP) analysis. (B) RFLP analysis of embryos injected with increasing dosages of vcla TALEN mRNA. Uncleaved bands represent efficient TALEN activity. (C) Summary of the range of mutations found at the vcla TALEN target locus in the F1 offspring of vcla TALEN-injected fish. Arrow denotes the mutation of the vcla mutant used in further experiments. (D) Sequence chromatograms from cDNA of wild-type vinculin (top) and of the vcla Δ8B mutant allele (bottom).