Skip to main content
. 2017 Aug 2;12(8):e0182278. doi: 10.1371/journal.pone.0182278

Fig 4. Generation of vinculin B-deficient zebrafish using CRISPR-Cas.

Fig 4

(A) Schematic representation of the endogenous vclb locus showing the CRISPR guide RNA target site in exon 1. (B) Sequence chromatograms of genomic DNA from non-injected control (top) and vclb CRISPR-injected embryos. The arrowhead denotes the expected CRISPR cleavage site (3 nucleotides upstream of the PAM site). (C) Summary of mutations found in the F1 offspring of vclb CRISPR-injected fish of either a wild-type (top) or vcla mutant (bottom) background. Arrows denote the mutation of the vclb mutant used in further experiments. (D) Amino acid sequence of vclb in wild-type (top) and vclb mutant (bottom). Asterisk denotes the premature stop codon found in the vinculin B coding sequence of vclb mutants.