Fig. 1.

MCF-7 express low expression of Nav1.5 and nNav1.5 but higher REST expression compared to MDA-MB-231. Relative mRNA expression level of Nav1.5 and nNav1.5 was measured using qRT-PCR where β-actin was used as housekeeping gene. a The expression of Nav1.5 in MDA-MB-231 normalised to MCF-7 cells. b The expression of nNav1.5 in MDA-MB-231 normalised to MCF-7 cells. c The expression of REST in MDA-MB-231 normalised to MCF-7 cells. Data were collected from n = 3 independent experiments, presented as mean ± SEM. Unpaired Student’s t test *p < 0.05, **p < 0.01, and *****p < 0.00001