Fig. 4.

TSA downregulated REST gene expression in MCF-7 cells. MCF-7 cells were treated with 10–10,000 ng/ml TSA for 24 h. Relative mRNA expression level of REST was measured using qRT-PCR where β-actin was used as housekeeping gene and normalised to untreated. Data were collected from n = 3 independent experiments, presented as mean ± SEM. Unpaired Student’s t test *p < 0.05, ***p < 0.001 and ****p < 0.0001