Fig. 9.

MMP2 and N-cadherin mRNA expression in MCF-7 cells was upregulated by TSA. Relative mRNA expression level was measured using qRT-PCR where β-actin was used as housekeeping gene. a Relative mRNA expression level of MMP2 normalised to untreated. b Gel electrophoresis images of qRT-PCR products of MMP2. c Relative mRNA expression level of N-cadherin normalised to untreated. d Gel electrophoresis images of qRT-PCR products of N-cadherin. For gel images, lane 1 untreated, lanes 2–4 100, 1000 and 10,000 ng/ml TSA, lane 5 non-template control. Data were collected from n = 3 independent experiments, presented as mean ± SEM. Unpaired Student’s t test *p < 0.05 and **p < 0.01