Table 3.
Proteins involved in proteolytic processes identified in the midgut of Ornithodoros moubata females before feeding (unfed) and at 48 h post-feeding (fed). (T) Gene identified in the intestinal transcriptome of O. moubata [29]
| Experimental group | Database | UniProt ID | Protein | emPAI Unfed | emPAI Fed | Molecular function |
|---|---|---|---|---|---|---|
| Unfed | EST_Acari | I3VR81 | Aminopeptidase N-10 | 0.31 | – | Metallopeptidase activity |
| Unfed | EST_Acari | B7PNL8 | Cysteine proteinase (T) | 2.34 | – | Cysteine-type peptidase activity |
| Unfed | mRNA_Om | B7P458 | Glutamate carboxypeptidase (T) | 0.04 | – | Carboxypeptidase activity |
| Unfed | NCBI_metazoa | G0WRZ9 | Papilin (T) | 0.02 | – | Metalloendopeptidase activity |
| Unfed | mRNA_Om | B7PKC5 | Sentrin/sumo-specific protease (T) | 0.05 | – | Endopeptidase activity |
| Fed | EST_Acari | A0A067R7D1 | ATP-dependent metalloprotease YME1L1 | – | 0.39 | Metalloendopeptidase activity |
| Fed | EST_Acari | E5SL05 | Cathepsin B (T) | – | 0.47 | Cysteine-type peptidase activity |
| Fed | EST_Acari | R4JNG8 | Cathepsin L (T) | – | 0.31 | Cysteine-type peptidase activity |
| Fed | mRNA_Om | Q5DNW1 | Furin-like convertase (T) | – | 0.04 | Serine-type endopeptidase activity |
| Fed | mRNA_Om | B4M1S6 | GJ18794 (T) | – | 0.07 | Serine-type endopeptidase activity |
| Fed | EST_Acari | XP_010742692 | Legumain isoform X2 (T) | – | 0.22 | Cysteine-type endopeptidase activity |
| Fed | mRNA_Om | B7P1K5 | Prenyl protease (T) | – | 0.09 | Metalloendopeptidase activity |
| Fed | mRNA_Om | E9FS53 | Putative uncharacterized protein (T) | – | 0.04 | Serine-type endopeptidase activity |
| Fed | mRNA_Om | Q6L7Z5 | Serine protease (T) | – | 0.07 | Serine-type endopeptidase activity |
| Unfed-Fed | EST_Acari | B7PJ08 | Aspartic protease (T) | 0.59 | 0.52 | Aspartic-type endopeptidase activity |
| Unfed-Fed | EST_Acari | A0A087TC24 | ATP-dependent zinc metalloprotease YME1-like protein | 0.24 | 0.27 | Metalloendopeptidase activity |
| Unfed-Fed | mRNA_Om | G3MKP9 | Carboxypeptidase (T) | 0.43 | 0.43 | Serine-type carboxypeptidase activity |
| Unfed-Fed | mRNA_Om | A0A087UZQ9 | Caspase-1 (T) | 0.25 | 0.2 | Cysteine-type endopeptidase activity |
| Unfed-Fed | EST_Acari | E7E820 | Cathepsin D2 | 0.59 | 0.35 | Aspartic-type endopeptidase activity |
| Unfed-Fed | EST_Acari | C6L6E2 | Cysteine protease (T) | 2.25 | 1.94 | Cysteine-type peptidase activity |
| Unfed-Fed | mRNA_Om | B4Q2G2 | GE16138 (T) | 1.47 | 0.33 | Cysteine-type endopeptidase activity |
| Unfed-Fed | EST_Acari | Q6PRC7 | Legumain-like protease (T) | 0.33 | 2.15 | Cysteine-type peptidase activity |
| Unfed-Fed | mRNA_Om | B7QLQ7 | Leucine aminopeptidase (T) | 0.13 | 0.2 | Metalloexopeptidase activity |
| Unfed-Fed | EST_Acari | B7PF28 | Longipain (T) | 0.62 | 0.44 | Cysteine-type endopeptidase activity |
| Unfed-Fed | EST_Acari | F0J8F6 | Metallopeptidase (T) | 0.41 | 0.44 | Metallopeptidase activity |
| Unfed-Fed | mRNA_Om | B7PDF5 | Prolyl endopeptidase (T) | 0.45 | 0.56 | Serine-type endopeptidase activity |
| Unfed-Fed | EST_Acari | A0A087UKQ1 | Retinoid-inducible serine carboxypeptidase (T) | 1.47 | 1.96 | Serine-type carboxypeptidase activity |
| Unfed-Fed | mRNA_Om | E2AUP0 | Serine protease HTRA2, mitocondrial (T) | 0.26 | 0.77 | Serine-type endopeptidase activity |
| Unfed-Fed | mRNA_Om | Q6U8A8 | Serine protease-like protein (T) | 2.87 | 2.87 | Serine-type endopeptidase activity |
| Unfed-Fed | mRNA_Om | B7PL27 | Signal peptide peptidase (T) | 0.25 | 0.47 | Aspartic-type endopeptidase activity |
| Unfed-Fed | mRNA_Om | B7PQH8 | Tripeptidyl-peptidase II (T) | 0.22 | 0.07 | Serine-type endopeptidase activity |
| Unfed-Fed | mRNA_Om | E2BH15 | Xaa-Pro aminopeptidase (T)1 | 1.23 | 0.16 | Metallopeptidase activity |