Table 7.
Proteins involved in the stress responses associated to blood digestion that were identified in the midgut of Ornithodoros moubata females before feeding (unfed) and at 48 h post-feeding (fed). (T) Gene identified in the intestinal transcriptome of O. moubata [29]
| Experimental group | Database | UniProt ID | Protein | emPAI Unfed | emPAI Fed | Molecular function |
|---|---|---|---|---|---|---|
| Antioxidants | ||||||
| Unfed | EST_Acari | B7Q8W6 | Alkyl hydroperoxide reductase, thiol specific antioxidant | 0.53 | – | Oxidoreductase activity |
| Unfed | mRNA_Om | B7QGB0 | Peroxidase (T) | 0.09 | – | |
| Unfed | mRNA_Om | B7PUM7 | Peroxinectin (T) | 0.1 | – | |
| Unfed | NCBI_metazoa | Q9GV35 | Peroxiredoxin (T) | 0.46 | – | |
| Unfed | EST_Acari | B7QN17 | Thioredoxin-dependent peroxide reductase (T) | 0.99 | – | |
| Fed | EST_Acari | T2FDK5 | Catalase (T) | – | 1.45 | |
| Fed | EST_Acari | A6N9S1 | Thioredoxin peroxidase (T) | – | 1.84 | |
| Unfed-Fed | EST_Acari | F0JAD0 | Dihydrolipoamide dehydrogenase | 2.44 | 0.32 | |
| Unfed-Fed | EST_Acari | Q2XW17 | Glutathione peroxidase (T) | 1.65 | 1.1 | |
| Unfed-Fed | EST_Acari | B7PUC4 | Superoxide dismutase [Cu-Zn] (T) | 4.58 | 2.15 | |
| Unfed-Fed | mRNA_Om | E2ASR9 | Thioredoxin-like protein 1 (T) | 1.68 | 0.17 | |
| Unfed-Fed | EST_Acari | A6NA14 | Truncated peroxiredoxin | 1.04 | 0.61 | |
| Unfed-Fed | mRNA_Om | B7PTG8 | Thioredoxin reductase (T) | 0.53 | 0.04 | |
| Chaperones | ||||||
| Unfed | EST_Acari | B7Q6Y2 | Chaperonin subunit | 0.58 | – | Protein folding |
| Unfed | EST_Acari | A0A097A1J8 | Heat shock 70 kDa protein | 18.61 | – | |
| Unfed | NCBInr | gi|557,767,195 | heat shock 70 kDa protein cognate 4-like | 0.2 | – | |
| Unfed | NCBI_metazoa | Q4ZJ79 | Heat shock cognate 70 protein (T) | 0.2 | – | |
| Unfed | EST_Acari | B7PEU9 | Heat shock protein (T) | 0.35 | – | |
| Unfed | EST_Acari | B7PIN1 | Heat shock protein 20.6 (T) | 0.27 | – | |
| Unfed | NCBI_metazoa | Q5I5Q6 | Heat shock protein 60 (T) | 0.23 | – | |
| Unfed | EST_Acari | B7QI01 | Hsp90 protein (T) | 2.82 | – | |
| Unfed | mRNA_Om | G3MRN7 | Putative uncharacterized protein | 0.27 | – | |
| Unfed | EST_Acari | B7P3Z6 | T-complex protein 1 subunit gamma (T) | 1.06 | – | |
| Unfed | NCBInr | E4WZX2 | Whole genome shotgun assembly, reference scaffold set | 0.08 | – | |
| Fed | EST_acari | F0 J987 | Endoplasmic reticulum glucose-regulated protein | – | 1.8 | |
| Fed | mRNA_Om | B4JT04 | GH23301 | – | 0.17 | |
| Fed | EST_acari | E4W3Z2 | Heat shock 70 kDa protein 5 (T) | – | 8.27 | |
| Fed | NCBInr | B4YTT9 | Heat shock protein 70–2 (T) | – | 0.19 | |
| Fed | EST_Acari | F0J8P3 | HSP70 family member (T) | – | 7.42 | |
| Fed | mRNA_Om | B7Q150 | Molecular chaperone | – | 0.1 | |
| Fed | mRNA_Om | G3MQW7 | Putative uncharacterized protein | – | 0.21 | |
| Fed | mRNA_Om | B7PN00 | TPR domain-containing protein (T) | – | 0.11 | |
| Fed | NCBI_metazoa | U4TVI5 | Uncharacterized protein | – | 0.1 | |
| Unfed-Fed | EST_Acari | B7PGQ2 | Calnexin | 0.51 | 0.67 | |
| Unfed-Fed | EST_Acari | Q68HD1 | Calreticulin | 1.4 | 0.73 | |
| Unfed-Fed | EST_Acari | B7PZ24 | Chaperonin complex component, TCP-1 delta subunit | 0.27 | 0.23 | |
| Unfed-Fed | EST_Acari | B7QJ21 | Chaperonin complex component, TCP-1 eta subunit | 0.23 | 1.67 | |
| Unfed-Fed | mRNA_Om | G6DGE9 | Chaperonin containing t-complex polypeptide 1 beta subunit | 4.89 | 6.06 | |
| Unfed-Fed | EST_Acari | B7PEV0 | Chaperonin subunit | 1.11 | 1.04 | |
| Unfed-Fed | EST_Acari | F0J8S6 | FKBP-type peptidyl-prolyl cis-trans isomerase | 0.44 | 0.26 | |
| Unfed-Fed | mRNA_Om | B4KRE5 | GI20465 | 1.34 | 0.91 | |
| Unfed-Fed | EST_Acari | A0A087TSI6 | GrpE protein homolog | 0.36 | 0.85 | |
| Unfed-Fed | mRNA_Om | B7PPJ5 | Grpe protein | 0.34 | 0.47 | |
| Unfed-Fed | EST_Acari | B7PWF5 | Heat shock protein 20.5 (T) | 3.55 | 0.47 | |
| Unfed-Fed | mRNA_Om | B7Q0B3 | Heat shock protein 70 (HSP70)-interacting protein (T) | 0.59 | 0.54 | |
| Unfed-Fed | NCBI_metazoa | M9WB33 | Heat shock protein 90 | 0.21 | 3.51 | |
| Unfed-Fed | EST_Acari | F0J8Z3 | Mitochondrial chaperonin Cpn60/Hsp60p | 2.85 | 1.16 | |
| Unfed-Fed | EST_Acari | B7PD56 | Peptidyl-prolyl cis-trans isomerase (T) | 4.59 | 0.66 | |
| Unfed-Fed | mRNA_Om | G3MKV9 | Putative uncharacterized protein | 1.37 | 1.42 | |
| Unfed-Fed | EST_Acari | A0A087UTZ9 | T-complex protein 1 subunit alpha (T) | 0.71 | 0.97 | |
| Unfed-Fed | mRNA_Om | B7PRH5 | T-complex protein 1 subunit delta (T) | 2.04 | 4.61 | |
| Unfed-Fed | EST_Acari | B7QC85 | Tumor rejection antigen (Gp96) | 1.29 | 1.32 | |
| Unfed-Fed | mRNA_Om | H9JT91 | Uncharacterized protein (T) | 2.64 | 6.15 | |
| Detoxification | ||||||
| Unfed | mRNA_Om | Q9Y1T8 | Cytochrome P450 4 W1 (T) | 0.37 | – | Heme binding |
| Unfed | mRNA_Om | B7P9Q4 | Cytochrome P450 (T) | 0.05 | – | |
| Fed | EST_Acari | B7P5J0 | Cytochrome P450 (T) | – | 0.74 | |
| Fed | mRNA_Om | B7QES0 | Cytochrome P450 (T) | – | 1.18 | |
| Fed | mRNA_Om | B7QJS5 | Cytochrome P450 (T) | – | 0.23 | |
| Unfed-Fed | mRNA_Om | B7PME9 | Cytochrome P450 (T) | 0.98 | 1.78 | |
| Unfed-Fed | mRNA_Om | B7PG84 | Glutathione S-transferase (T) | 21.84 | 2.09 | Transferase activity |
| Unfed-Fed | EST_Acari | B7PR98 | Glutathione S-transferase (T) | 4.24 | 4.97 | |
| Unfed-Fed | mRNA_Om | B7Q917 | Glutathione S-transferase (T) | 0.8 | 0.69 | |
| Unfed-Fed | mRNA_Om | B7PRA0 | Glutathione S-transferase (T) | 2.54 | 0.33 | |
| Unfed-Fed | mRNA_Om | B7QMI2 | Glutathione S-transferase (T) | 9.09 | 0.49 | |