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Observations made in bovine chondrocytes were further verified in the same type of cells in humans. Articular chondrocytes were isolated from the ankle cartilage of an amputated patient. Passage 2 cells were treated with DAMPs and/or Fn-f (a) or IL-1β (b) for 24 hrs. Medium samples were analyzed for MMP-3 expression with Western blotting. Relative intensity of protein bands on each blot was measured and plotted, respectively.
