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. 2017 Aug 2;12(8):e0182373. doi: 10.1371/journal.pone.0182373

Fig 5. c-REL knockout leads to significantly decreased expression levels of NF-κB family member and cell cycle-associated genes.

Fig 5

A: qPCR analysis showing significantly decreased mRNA levels of NF-κB family members RELA, NFKB1 (p50), NFKB2 (p52), IKBKE and TBK1 in c-REL knockout cells compared to wildtype cells. B-C: Expression levels of cell cycle-related c-REL target genes A20, BCL2, BCL-XL and TGFB1 and c-REL target genes MYC and ICAM-1 were significantly decreased in c-REL knockout cells in comparison to HeLa Kyoto wildtype cells. D: Western blot analysis validated the reduced expression levels of RELA and A20 in c-REL-/- cells in comparison to wt on protein level. WB were performed after TNFα-dependent stimulation of c-REL-/- and c-REL+/+ cells. E: Immunocytochemistry revealed a strongly decreased protein amount of ICAM in c-REL-/- cells in comparison to wt.