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. 2017 Aug 2;12(8):e0182398. doi: 10.1371/journal.pone.0182398

Table 2. Primer pairs and annealing conditions used for gene expression analyses.

Name Sense primer Antisense primer Product size Annealing temperature
CDX2 CTTTCCTCCGGATGGTGATA AGCCAAGTGAAAACCAGGAC 113 bp 63°C
OCT4 GTTTTGAGGCTTTGCAGCTC CTCCAGGTTGCCTCTCACTC 185 bp 63°C
NANOG AAACAACTGGCCGAGGAATA AGGAGTGGTTGCTCCAAGAC 194 bp 63°C
GAPDH TTCAACGGCACAGTCAAGG ACATACTCAGCACCAGCATCAC 119 bp 63°C
YWHAZ GCATCCCACAGACTATTTCC GCAAAGACAATGACAGACCA 120 bp 63°C