Table 2. Primer pairs and annealing conditions used for gene expression analyses.
Name | Sense primer | Antisense primer | Product size | Annealing temperature |
---|---|---|---|---|
CDX2 | CTTTCCTCCGGATGGTGATA | AGCCAAGTGAAAACCAGGAC | 113 bp | 63°C |
OCT4 | GTTTTGAGGCTTTGCAGCTC | CTCCAGGTTGCCTCTCACTC | 185 bp | 63°C |
NANOG | AAACAACTGGCCGAGGAATA | AGGAGTGGTTGCTCCAAGAC | 194 bp | 63°C |
GAPDH | TTCAACGGCACAGTCAAGG | ACATACTCAGCACCAGCATCAC | 119 bp | 63°C |
YWHAZ | GCATCCCACAGACTATTTCC | GCAAAGACAATGACAGACCA | 120 bp | 63°C |