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. 2017 Aug 1;9:57. doi: 10.1186/s13195-017-0279-1

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Immunocytochemistry in primary neurons to elucidate the MAO-B location in relation to the synapse. Primary hippocampal neurons were stained with Phalloidin to visualize fibrillar actin and the neuronal structure (white), DAPI to visualize nuclei (blue), anti-NMDAr2B to visualize the postsynaptic side of glutamatergic synapses (green) and anti-MAO-B (red). a Low-magnification overview imaged with a 20× objective, to visualize that most or all of the pyramidal neurons in our cell cultures contain NMDAr2B. b High-magnification image taken with a 60× oil immersion objective. Inset shows a zoomed part of the large image that was enriched with a high density of NMDAr-containing dendritic spines and MAO-B staining on the opposing axons. The intensity of the red channel in the inset was enhanced using image J (Color figure online)