Fig 2. Functional assessment of HSCs from young healthy MSH2-/- mice.

(A). 2x10⁶ whole BM pooled from 6–8 weeks old WT (n = 3) or MSH2-/- (n = 3) donor mice (CD45.2) into irradiated recipients (CD45.1, n = 5 per group). 8 weeks after transplantation, donor chimerism in the peripheral blood was analyzed and quantitated. Similar results were obtained in three independent experiments. (B). 1x10³ sorted LSK cells from 6–8 weeks old WT or MSH2-/- (n = 3 each) donor mice (CD45.2) along with 2x10⁵ BoyJ BM cells were transplanted into irradiated recipients (CD45.1, n = 10 per group). 8 and 12 weeks after transplantation, donor chimerism in the peripheral blood was analyzed and quantitated. Similar results were obtained in two independent experiments. (C). BM from 6–8 weeks old WT and MSH2-/- mice (CD45.2) was harvested and mixed with WT BM (CD45.1) at 1:1 ratio and transplanted into lethally irradiated WT mice (CD45.1, n = 10 per group). 8 weeks after transplantation, donor chimerism in the peripheral blood was analyzed and quantitated. Similar results were obtained in two independent experiments. Error bars indicate the SD, and significance was determined by a two-tailed t test. Asterisk, P<0.05. (D). Survival curve of the recipients of the experimental group described in (C).