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. 2017 Aug 2;12(8):e0181190. doi: 10.1371/journal.pone.0181190

Fig 3. GLP-1 fails to rescue apoptosis in ENPP1 transfected cells.

Fig 3

Apoptosis was assessed by caspase 3/7 activity, in INS-1E (panel A) and βTC-1 (panel B) cells, either transfected with neo or ENPP-1 gene variant. Cells were treated with staurosporine (0.25 μmol/l, 2 hours) in the absence or presence of 100 nmol/l GLP-1. Treatment with GLP-1 significantly decreased apoptosis induced by staurosporine in INS-1E-neo and in βTC-1-neo cells but not in INS-1E-ENPP1 and in βTC-1-ENPP1 cells. Values are expressed as means ± SD in INS-1E (n = 7) in βTC-1 (n = 8) independent experiments, each comprising three wells. (* p<0.05 compared with untreated INS-1E-neo or βTC-1-neo cells; § p<0.005 compared with INS-1E-neo or βTC-1-neo cells treated with staurosporine; ° p<0.05 compared with untreated INS-1E-ENPP1 or βTC-1-ENPP1; # p< 0.05 compared with INS-1E-neo or βTC-1-neo cells treated with staurosporine; $ p< 0.05 compared with INS-1E-neo or βTC-1-neo cells exposed to GLP1).