Fig 4. Memory phenotype of vaccine-induced SIV-specific CD8+ T-cells in PBMC at the time of SIV challenge.
(A) Gating strategy used to delineate fully differentiated effector memory (TEM2), transitional memory (TEM1), or central memory (TCM) subsets within tetramer+ CD8+ T-cells in peripheral blood. Mamu-A*01 tetramers folded with the following peptides were analyzed: Gag CM9 (B), Vif VL10 (C), and Tat SL8 (D). Mamu-A*02 tetramers folded with the following peptides were analyzed: Gag GY9 (E), Vif WY8 (F), and Nef YY9 (G). Groups 1, 2, 3, and 4 are color coded in pink, green, beige, and black, respectively. Lines represent medians, and each symbol corresponds to one vaccinee.