Fig 5. Vaccine-induced Env-binding antibodies in Groups 1 and 2.

Binding antibodies to Env were measured by ELISA using plate-bound gp140 at multiple time points during the vaccine phase. Straight 1:200 dilutions of plasma from each of the vaccinees in Group 1 (A) and Group 2 (B) were used for this analysis. Monkey r10062 was not primed with EP rDNA since it was enrolled in Group 1 shortly before the rAd5 boost as a replacement for a rhesus macaque that died unexpectedly. C) Log-transformed endpoint titers of vaccine-induced Env-binding antibodies in Groups 1 and 2 at the time of SIV challenge. As a reference, these values were plotted alongside the endpoint titers of Env-binding antibodies in four rhesus macaques that had been infected with SIVmac239Δnef for 28 weeks as part of a previous experiment (43).