Figure 2.

RhoGDIα mediated p65 promoting BC migration.

(A & B) The indicated cell extracts were subjected to Western blot to determine protein expression of p65, RhoGDIα, RhoA, and Rac1. GAPDH was used as a loading control. (C) RhoGDIα knockdown constructs were stably transfected into T24T(shp65-1) cells. The stable transfectants were identified by Western blotting. (D) The migration and invasion abilities of T24T(shp65/shRhoGDIα) and their nonsense control T24T(shp65/nonsense) were determined as described in “Materials and Methods” section. Bars represent mean ± SD from three independent experiments. Student's t test was utilized to determine the P value, and the symbol (♣) indicates a significant increase as compared with nonsense control cells (P < .05) (E). (F) The invasion rate was normalized with the insert control according to the manufacturer's instruction. The bars represent mean ± SD from three independent experiments. Student's t test was utilized to determine the P value, P > .05.