Figure 3.

p65 promoted degradation of RhoGDIα protein.

(A & B) T24T(shp65-1), T24T(shp65-2) vs. T24T(nonsense) or MEF(p65−/−), MEF[p65−/−(p65)] vs. MEF(WT) cells were extracted for total RNA with Trizol reagent. RT-PCR assay was used to determine RhoGDiα mRNA expression. β-Actin was used as an internal control. (C) The indicated cells were pretreated with proteasome inhibitor MG132 for 10 hours and were then followed by treatment of cells with CHX for the indicated times. The cell extracts were subjected to Western blot, and GAPDH was used as a protein loading control.