Skip to main content
. Author manuscript; available in PMC: 2017 Aug 3.
Published in final edited form as: Science. 2016 Jun 2;353(6294):45–50. doi: 10.1126/science.aaf7865

Figure 2. Activity of asPARPs 1, 2, and 3 with a clickable NAD+ analog.

Figure 2

(A) Chemical structure of the bi-functional NAD+ analog 8-Bu(3-yne)T-NAD+ with the clickable analog sensitivity-inducing, alkyne-containing R group highlighted in red.

(B) Schematic illustrating asPARP activity-dependent, click chemistry-mediated covalent attachment of fluorophores, biotin, or agarose resin to 8-Bu(3-yne)T-ADP-ribosylated proteins.

(C) Automodification reactions with wild-type or analog-sensitive PARP-1, PARP-2, and PARP-3 analyzed by Western blotting for ADP-ribose (top) or click chemistry-based in-gel fluorescence (bottom).