Figure 3. Expression of IDH2^K413Q promotes a transformation permissive phenotype.

(a) Fifty MCF7-shIDH2-IDH2^WT, MCF7-shIDH2-IDH2^K413R, or MCF7-shIDH2-IDH2^K413Q cells were plated on a 6-well cell culture plate for 14 days before colonies were stained using crystal violet. (b) Quantification of colony numbers (n=4). (c) Two hundred and fifty MCF7-shSIRT3-PCDH, MCF7-shSIRT3-IDH2^K413R and MCF7-shSIRT3-IDH2^K413Q cells were plated on a 6-well cell culture plate for 14 days before colonies were stained using crystal violet. (d) Quantification of colony numbers (n=4). (e) Tumors from immunodeficient mice injected with five million MCF7-shIDH2-IDH2^WT, MCF7-shIDH2-IDH2^K413R, or MCF7-shIDH2-IDH2^K413Q cells were harvested and weighed (n=5). (f) Tumor growth was measured starting at day six of tumor incidence (n=5). Results are the average of five biological replicates, and error bars represent one standard deviation. * indicates p<0.05, ** indicates p<0.01, comparing to WT by two-tailed unpaired t-test using Prism 6.0.