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Immunohistochemistry (IHC) and real-time PCR of genes expressed in fibrocartilage and bone. A) IHC of type I collagen in WT (left panels) and bgn −/−, fmod−/− deficient condyles (right panels) treated without (top panel) or with (bottom panels) BMP-2. B) Real-time PCR of type I collagen (Col1), C) Runx2 D) osterix (Osx) and E) Wisp1 mRNA in WT and Bgn−/−Fmod−/− condyles treated with or without BMP-2. *p<0.05, #p<0.01
