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. 2017 Aug 3;8:1347. doi: 10.3389/fpls.2017.01347

FIGURE 1.

FIGURE 1

IFR1 localizes to the cytosol in Chlamydomonas reinhardtii cells. Laser scanning confocal microscopy detection of subcellular localization of the mVenus (yellow) fluorescent reporter fused to N- or C-terminus of IFR1 (N/C). A cell line expressing mVenus in the cytosol (Cyto, Lauersen et al., 2015) and the parental strain (PCS) served as controls. Individual imaging channels are presented, YFP: mVenus reporter signal in the yellow range, Chlorophyll: autofluorescence of chlorophyll visualized in the red range and used to orient the cells, Overlay: YFP and Chloro channel overlay, DIC: differential interference contrast. Scale bars represent 5 μm.