FIGURE 7.

The protective effects of ginsenoside Rb1 on TNF-α-induced injury in HUVECs are p38 and JNK-pathway dependent. HUVECs were incubated with ginsenoside Rb1 (20 μg/mL) for 8 h before treatment with TNF-α for 24 h. (A) The expression levels of phosphorylated and total JNK and p38 were detected by western blot. (C,E) Ginsenoside Rb1 suppressed NF-κB nuclear translocation and regulated apoptosis-related protein expression by inhibiting the JNK and p38 pathway. (B,D,F) Densitometric analysis was performed to quantify protein expression levels. Data were expressed as the mean ± SD of three independent experiments. ^##P < 0.01, vs. control group; ^∗P < 0.05, ^∗∗P < 0.01 vs. TNF-α treatment group. ^$P < 0.05, ^$$P < 0.01, vs. TNF-α and ginsenoside Rb1 groups.