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. 2017 Aug 2;7:7104. doi: 10.1038/s41598-017-07005-1

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Generation of cisplatin-resistant EOC cells. (A) Experimental design used in the generation of cisplatin-resistant (MI-res) cells. Cells, plated at high confluence (1), were exposed for 2 hours to a cisplatin dose 10-fold higher than their calculated IC50 (2). After a suspension period (3), recovered cells were subjected again to step 1–3, for additional 20 cycles and then their cisplatin sensitivity was assessed again. (B–D) Cisplatin (B), Taxol (C) and Doxorubicin (D) dose response curves using parental and MI-res cells, as indicated. Results are expressed as percentage of viable cells respect to untreated cells. IC50 (half maximal inhibitory concentrations) are reported (n = 3 biological replicates each performed in triplicate). In the lower table, the IC50 calculated for each drug and cell line is shown. In (B,C and D), the difference between parental cells and each MI-res clone is reported. Statistical significance was determined by repeated-measures analysis of one-way-ANOVA test. *p < 0.05; **p < 0.01; ***p < 0.001; ns = not significant.