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. 2017 Aug 2;7:7104. doi: 10.1038/s41598-017-07005-1

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Expression of coding and non-coding genes does not identify common gene expression alteration in MI-res cells. (A) Heat map of supervised clustering analyses evaluating the expression of coding and long non-coding RNAs in parental and MI-res cells, as indicated. (B) Venn diagram showing the genes differentially expressed between parental and MI-res cells, in each cell line. Few genes were commonly altered in the three models and none in the same direction (i.e. always increased or decreased in MI-res vs parental cells). (C) Heat map of supervised clustering analyses evaluating the expression of microRNAs in parental and MI-res cells, as indicated. (D) Venn diagram showing the microRNAs differentially expressed between parental and MI-res cells in each cell line. Only 3 microRNAs were commonly altered in the three models and none in the same direction (i.e. always increased or decreased in MI-res vs parental cells). For both coding and non coding gene expression analyses, 2 samples for each parental and 2 for each MI-res cell line were analyzed.