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. 2017 Aug 2;7:7168. doi: 10.1038/s41598-017-07149-0

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Expression analysis of myosin heavy chain isoforms in neonatal skeletal muscle. Expression levels of MyHC isoforms were measured by qPCR in the gastrocnemius-plantaris-soleus (GPS) muscle complex at postnatal day 7 (P7) of Vgll2 ^+/+ and Vgll2 ^−/− mice (n = 8). Myh7 (encoding MyHCI), Myh2 (MyHCIIa), Myh1 (MyHCIIx), Myh4 (MyHCIIb), Myh7b (slow-tonic MyHC), Myh3 (embryonic fast isoform, MyHC-emb), and Myh8 (perinatal fast isoform, MyHC-pn) expression levels were examined. For comparison, the expression level of these genes in Vgll2 ^+/+ mice was arbitrarily set at 1. Data are presented as mean ± SEM. *P < 0.05 vs. Vgll2 ^+/+ muscles.