Figure 5.

Physical interaction analysis between endogenous Vgll2 and TEAD1 in skeletal muscles at the neonatal stage. (a) Immunoprecipitation was conducted with the anti-Vgll2 antibody followed by Western blot analysis to detect TEAD1, TEAD4 and Vgll2 in GPS muscle extracts from Vgll2 ^+/+ and Vgll2 ^−/− mice at P7. (b) Immunoprecipitation was conducted with an anti-TEAD1 antibody followed by Western blot analysis to detect Vgll2 and TEAD1 in P7 GPS muscle extracts. (c) Immunoprecipitation was conducted with an anti-TEAD4 antibody followed by Western blot analysis to detect Vgll2 and TEAD4 in P7 GPS muscle extracts. Control samples were GPS muscle lysates incubated without anti-Vgll2 or -TEAD1 antibody (no-Ab). Uncropped original images are shown in Supplementary information.