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. 2017 Aug 2;7:7116. doi: 10.1038/s41598-017-07559-0

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Selective silencing and overexpression of Kaiso in endothelial cells. HUVECs (A,B) and HMEC-1s (D,E) were transfected with NC-siRNA or Kaiso-siRNA1 and silencing efficiency was evaluated by quantitative real-time PCR (A and D) and Western blot (B and E) at 48, 60 or 72 h post transfection. HUVECs (C) and HMEC-1s (F) were transfected with pCDNA3.1 or pCDNA3.1-Kaiso and Kaiso expression was evaluated by Western blot at 48 and 72 h after transfection. GAPDH served as a loading control. Values are presented as mean ± SD, **p < 0.01, compared with blank, n = 3.