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. 2017 Aug 2;7:7121. doi: 10.1038/s41598-017-07479-z

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Internalization of FGF1 and scFvD2-Fc occurs via clathrin mediated endocytosis. Internalization of FGF1.myc (a) and scFvD2-Fc (b) into U2OSR1 cells was studied in the presence of inhibitors of dynamin (Dynasore; 80 µM) and clathrin (Pitstop2; 30 µM). Densitometric quantification of FGF1.myc and scFvD2-Fc internalization was performed with ImageJ from 3 independent experiments. Fraction of internalized FGF1.myc and scFvD2-Fc after treatment with inhibitors is represented as a percentage of untreated control. Error bars represent standard deviation. Cropped blots were displayed, full size blots are included in Supplementary Information.