Figure 6.

P25/LPS induces Ifit2. Raw264.7 cells were incubated for the indicated times in DMEM + 10% FBS supplemented with P25 (128 µg/ml), LPS (10 ng/ml), or P25/LPS (nominal doses of 128 µg/ml, for TiO₂ NP, and 40 pg/ml, for LPS). Control cells (−) were incubated in plain DMEM + 10% FBS. (A) After 1 or 6 h, the activation of STAT1 was evaluated through WB analysis. A representative experiment, performed twice with comparable results, is shown. (B) After 6 or 24 h Ifit2 expression was assessed by RT-PCR. Data are means of two independent experiments, each performed in duplicate, with SD shown. *, **, ***, p < 0.05, p < 0.01, or p < 0.001 vs. cells treated with P25 at the same experimental time; ^###, p < 0.001 vs. cells treated with LPS at the same experimental time, as evaluated by two-tailed t-test for unpaired data.